IN VITRO ASSESSMENT OF BIOPESTICIDE BACILLUS THURINGIENSIS VAR. KURSTAKI HD- 1 EFFECTIVENESS ON PHYTOPHTHORA PALMIVORA, AGENT OF COCOA BLACK POD ROT IN CÔTE D’IVOIRE

  • Gadji Alahou Andre Gabaze Laboratoire des Sciences de l’Environnement, UFR des Sciences et Gestion de l’Environnement, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
  • Yapo Ossey Bernard Laboratoire des Sciences de l’Environnement, UFR des Sciences et Gestion de l’Environnement, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
  • Abo Kouabenan Laboratoire de Phytopathologie et de Biologie Végétale, Ecole Supérieure d’Agronomie, Institut National Polytechnique HOUPHOUET-BOIGNY, Yamoussoukro, Côte d’Ivoire
  • Coulibaly Klotioloma Laboratoire de phytopathologie, Centre National de Recherche Agronomique (CNRA), Station de recherche de Divo, Côte d’Ivoire
  • Kebe Boubacar Ismael Laboratoire de phytopathologie, Centre National de Recherche Agronomique (CNRA), Station de recherche de Divo, Côte d’Ivoire
  • Gnepe Jean Robert Laboratoire de bioconversion des eaux usées et de boues d’épuration en produits à haute valeur ajoutée, INRS-ETE, Université de Québec, Canada
  • Tyagi Rajeshwar Dayal Laboratoire de bioconversion des eaux usées et de boues d’épuration en produits à haute valeur ajoutée, INRS-ETE, Université de Québec, Canada

Abstract

Phytophthora Palmivora is responsible for the cocoa black pod rot in Côte d'Ivoire, with production losses varying from 20 to 40% depending on the regions. Effectiveness of the environmentally friendly biopesticide Bacillus thuringiensis var. kurstaki HD-1 was evaluated in vitro on the pathogen. The medium pea supercooled has been incorporated with the bioproduct at different concentrations: C1 = 100%, C2 = 50%, C3 =25%, C4 = 12.5% and C5 = 6.25%. This medium into Petri dishes was inoculated either by spreading, with 0.1 mL of P. palmivora inoculum at103 zoospores /mL, or with a mycelial disc of P. palmivora calibrated at 6 mm diameter in the center of the box. The results showed that the germination of zoospores was inhibited at the rate of 100% in the presence of the concentrations C1 and C2. This rate was 74.82 ± 21.53%, 73.17 ± 20.88% and 59.12 ± 39.05%, respectively, for C3, C4 and C5. The efficacy of the bioproduct is revealed by a significant reduction of mycelia growth ranging between 57.77± 2.4 to 100% in the presence of the concentrations C5 to C1. The IC50 of germination was 3.36% of concentration and the IC90 was 43.22%; while those which inhibit 50% and 90% of mycelial growth were 5.67% and 77.94% respectively. The biopesticide Btk HD-1 has a proven fungistatic effect against P. palmivora. Its effectiveness increases with its concentration. So it can be a valuable component of an integrated cocoa black pod management.

Downloads

Download data is not yet available.
Published
2015-07-30
How to Cite
Gabaze, G. A. A., Bernard, Y. O., Kouabenan, A., Klotioloma, C., Ismael, K. B., Robert, G. J., & Dayal, T. R. (2015). IN VITRO ASSESSMENT OF BIOPESTICIDE BACILLUS THURINGIENSIS VAR. KURSTAKI HD- 1 EFFECTIVENESS ON PHYTOPHTHORA PALMIVORA, AGENT OF COCOA BLACK POD ROT IN CÔTE D’IVOIRE. European Scientific Journal, ESJ, 11(21). Retrieved from https://eujournal.org/index.php/esj/article/view/6001