Inhibition Of TNF-α Release by D : B- Friedo - Olean -5- En- 3α -Ol in Human Monocytes

  • Andriamampianina Tianarilalaina Tantely Laboratoire de Pharmacologie Générale, de Pharmacocinétique et de Cosmétologie, Faculté des Sciences, Université d’Antananarivo, MADAGASCAR
  • Randrianavony Patricia Laboratoire de Pharmacologie Générale, de Pharmacocinétique et de Cosmétologie, Faculté des Sciences, Université d’Antananarivo, MADAGASCAR
  • Rasoanaivo Herilala Lea Laboratoire de Chimie des Substances Naturelles et de Chimie Organique Biologique, Faculté des Sciences, Université d’Antananarivo, MADAGASCAR
  • Pr Randimbivololona Fanantenanirainy Laboratoire de Pharmacologie Générale, de Pharmacocinétique et de Cosmétologie, Faculté des Sciences, Université d’Antananarivo, MADAGASCAR

Abstract

This study was focused on the release of TNF- α by cultured monocytes of human blood in the absence and presence of D : B Friedo - olean - 5 -en- 3α -ol (DBFO). The human monocytes were isolated from the peripheral blood mononuclear cells (PBMC). The release of TNF-α by monocytes was induced by the addition of lipopolysaccharide (LPS) to the culture medium. ELISA Sandwich's method was used to the dosing of TNF- α concentration. The release of TNF-α is inhibited by DBFO. In the absence of DBFO, TNF- α concentration is 3.86 ng/ml versus 3.77 ; 3.2 ; 2.79 and 0.89 ng/ml in the presence of DBFO at concentrations 1 , 3, 10 and 30 μg/ml respectively ( p <0.05) .

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Published
2016-10-31
How to Cite
Tantely, A. T., Patricia, R., Herilala Lea, R., & Fanantenanirainy, P. R. (2016). Inhibition Of TNF-α Release by D : B- Friedo - Olean -5- En- 3α -Ol in Human Monocytes. European Scientific Journal, ESJ, 12(30), 166. https://doi.org/10.19044/esj.2016.v12n30p166