Biosynthesis of Silver Nanoparticles using Ajuca ive Leaf Extract and Assessment of their Activity on E. coli and Streptococcus bacteria
Keywords:
Ajuca ive Leaf, Silver Nanoparticles, Antibacterial Activity, E. coli
Abstract
Silver nanoparticle synthesis from plant extracts has been widely used in medicine, particularly as an antibacterial agent. In the present study, Ajuca ive leaf extract was performed using an aqueous solution tested for its phytochemical components. The results of the phytochemical analysis of Ajuca ive leaf extract had alkaloids, carbohydrates, proteins, flavonoids, phenols, saponins, and coumarins. Synthesis of silver nanoparticles was performed using Ajuca ive leaf extract with a 1 mM solution of silver nitrate. The synthesized silver nanoparticles were characterized with UV-visible spectroscopy, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), and Fourier Transform Infra-Red (FTIR) spectrum. SEM analysis revealed the size of the AgNPs of 36 nm, 55 nm, 70 nm, 100, and 300 nm. The EDX study showed that the optical absorption peak was detected at 3 keV, the characteristic peak for the absorbed metallic silver nanoparticles. FTIR analysis identified the possible functional group involved in reducing silver metal ions into silver nanoparticles. In addition, the antibacterial activity of synthesized silver nanoparticles was also examined and the results showed good antibacterial activities against E. coli and Streptococcus bacteria.
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4. Gurunathan, S., Han, J., Park, J. H., & Kim, J. H. (2014). A green chemistry approach for synthesizing biocompatible gold nanoparticles. Nanoscale research letters, 9(1), 248. https://doi.org/10.1186/1556-276X-9-248
5. Gurunathan, S., Park, J. H., Han, J. W., & Kim, J. H. (2015). Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy. International journal of nanomedicine, 10, 4203–4222 https://doi.org/10.2147/IJN.S83953.
6. Chen, Y., Wang, C., Liu, H., Qiu, J., & Bao, X. (2005). Ag/SiO2: a novel catalyst with high activity and selectivity for hydrogenation of chloronitrobenzenes. Chemical communications (Cambridge, England), (42), 5298–5300. https://doi.org/10.1039/b509595f
7. Li, Z., Lee, D., Sheng, X., Cohen, R. E., & Rubner, M. F. (2006). Two-level antibacterial coating with both release-killing and contact-killing capabilities. Langmuir : the ACS journal of surfaces and colloids, 22(24), 9820–9823. https://doi.org/10.1021/la0622166
8. Setua, Palash & Chakraborty, Anjan & Seth, Debabrata & Bhatta, Umananda & Satyam, P. & Sarkar, Nilmoni. (2007). Synthesis, Optical Properties, and Surface Enhanced Raman Scattering of Silver Nanoparticles in Nonaqueous Methanol Reverse Micelles. Journal of Physical Chemistry C - J PHYS CHEM C. 111. 10.1021/jp067475i
9. Khatoon, A., khan, F., Ahmad, N., Shaikh, S., Mohd, S., Rizvi, D., Shakil,S., Ai-Qahtani,M.H., Abuzenadah, A. M,. Tabrez, S., Ahamed, A.B.F., Alafnan,A., Islam, H., Iqbal, D and Dutta, R. (2018). Silver nanoparticles from leaf extract of Mentha piperita: Eco-friendly synthesis and effect on acetylcholinesterase activity. Life Sciences, 209, 430-434. https://doi.org/10.1016/j.lfs.2018.08.046
10. Mukherjee, P., Ahmad, A., Mandal, D., Senapati, S., Sainkar, S. R., Khan, M. I., Pasricha, R., & Sastry, M. (2001). Fungus-Mediated Synthesis of Silver Nanoparticles and Their Immobilization in the Mycelial Matrix: A Novel Biological Approach to Nanoparticle Synthesis. Nano Letters, 1(10), 515-519. https://doi.org/10.1021/nl0155274
11. Kalishwaralal, K., Deepak, V., Ramkumarpandian, S., Nellaiah, H. and Sangiliyandi, G. (2008) Extracellular Biosynthesis of Silver Nanoparticles by the Culture Supernatant of Bacillus licheniformis. Materials Letters, 62, 4411-4413. https://doi.org/10.1016/j.matlet.2008.06.051
12. Gurunathan, S., Kalishwaralal, K., Vaidyanathan, R., Venkataraman, D., Pandian, S. R., Muniyandi, J., Hariharan, N., & Eom, S. H. (2009). Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Colloids and surfaces. B, Biointerfaces, 74(1), 328–335. https://doi.org/10.1016/j.colsurfb.2009.07.048
13. Akhtar, Mohd Sayeed & Panwar, Jitendra & Yun, Yeoung-Sang. (2013). Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustainable Chemistry & Engineering. 1. 591-602. https://www.researchgate.net/publication/278785701.
14. Shankar, S. S., Rai, A., Ahmad, A., & Sastry, M. (2004). Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. Journal of colloid and interface science, 275(2), 496–502. https://doi.org/10.1016/j.jcis.2004.03.003.
15. Iravani, S. (2011). Green synthesis of metal nanoparticles using plants. Green chemistry, 13(10), 2638-2650. https://www.researchgate.net/publication/221670104.
16. Rai, M., & Yadav, A. (2013). Plants as potential synthesiser of precious metal nanoparticles: progress and prospects. IET nanobiotechnology, 7(3), 117–124. https://doi.org/10.1049/iet-nbt.2012.0031
17. Dubey, M., Bhadauria, S., and Kushwah, B. (2009). Green synthesis of nanosilver particles from extract of Eucalyptus hybrid (safeda) leaf. Degest Journal of nanometer biostruct, 4, 537-543.
18. Huang, J., Li, Q., Sun, D., Lu, Y., Su, Y., Yang, X., Wang, H., Wang, Y., Shao, W., He, N., Hong, N.J. and Chen, C. (2007) Biosynthesis of Silver and Gold Nanoparticles by Novel Sundried Cinnamomum camphora Leaves. Nanotechnology, 18, 105104-105115.
https://doi.org/10.1088/0957-4484/18/10/105104.
19. Sehnal, K., Ozdogan,Y., Stankova, M., Tothova, Z., Uhlirova, D., Vsetickova, M., Hosnedlova, B., Kepinska, M., Ruttkay-Nedecky, B., Fernandez, C., Hari, V., Sochor, J., Milnerowicz, H and Kizek, R. 2019. Effect of silver nanoparticles (AgNPs) prepared by green synthesis from sage leaves (salvia officinalis) on maize chlorophyll content. In Proceedings of 11th Nanomaterials international conference 2019 (NANOCON 2019): research and applications, 16-18 October 2019, Brno, Czech Republic. Ostrava: Tanger Ltd, pages 457-462. Available from: https://doi.org/10.37904/nanocon.2019.8521
20. Miara, M.D., Hammou, M.A. & Aoul, S.H. Phytothérapie et taxonomie des plantes médicinales spontanées dans la région de Tiaret (Algérie). Phytothérapie 11, 206–218 (2013). https://doi.org/10.1007/s10298-013-0789-3
21. Boran, Ni & Dong, X & Fu, J & Xingbin, Y & Longfei, L & Zhenwen, X & Yang, Z & Xue, D & Yang, C & Ni, J.(2015). Phytochemical and Biological Properties of Ajuga decumbens (Labiatae): A Review. Tropical Journal of Pharmaceutical Research. 14. 1525. 10.4314/tjpr.v14i8.28
22. Coll J. (2002). NMR shift data of neo-clerodane diterpenes from the genus Ajuga. Phytochemical analysis : PCA, 13(6), 372–380. https://doi.org/10.1002/pca.671
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Published
2025-01-28
How to Cite
Sasi, M. M., Hribesh, S. O., Eshkourfu, R. O., & Amara, R. O. (2025). Biosynthesis of Silver Nanoparticles using Ajuca ive Leaf Extract and Assessment of their Activity on E. coli and Streptococcus bacteria. European Scientific Journal, ESJ, 37, 373. Retrieved from https://eujournal.org/index.php/esj/article/view/19036
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ESI Preprints
Copyright (c) 2025 Mariam Mohammed Sasi, Samira Omar Hribesh, Rabia Omar Eshkourfu, Rokaya Omar Amara
This work is licensed under a Creative Commons Attribution 4.0 International License.
