Water, Sanitation and Hygiene in Ebola Preparedness and Response in South Sudan, Uganda and the Democratic Republic of the Congo: A Scoping Review
Abstract
Water, sanitation and hygiene (WASH) services are fundamental to infection prevention and control during Ebola virus disease outbreaks. However, their contribution to outbreak preparedness and response remains insufficiently synthesised, particularly in fragile and outbreak-prone settings. This scoping review examined the importance of WASH in Ebola preparedness and response in Uganda, the Democratic Republic of the Congo (DRC) and South Sudan. Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). Literature published between 2014 and 2026 was searched through PubMed, African Index Medicus, Google Scholar, African Journals Online (AJOL) and the World Health Organization Institutional Repository for Information Sharing (IRIS). Peer-reviewed and grey-literature sources addressing WASH, infection prevention and control, preparedness or response to Ebola in the three countries were considered. The evidence was charted and synthesised thematically. The searches displayed 1,963 results, of which 861 were captured for title, abstract, summary, or full-text screening. After eligibility assessment and cross-platform deduplication, 41 unique sources were included. Six themes emerged: WASH infrastructure and service readiness; infection prevention and control preparedness; healthcare-worker preparedness; community engagement and risk communication; cross-border surveillance and institutional coordination; and institutional, financial and logistical constraints. Reliable water supplies, sanitation facilities, handwashing stations, environmental cleaning and waste-management systems supported effective infection-control practices. Nevertheless, preparedness was weakened by inadequate infrastructure, insufficient protective supplies, limited practical training, weak maintenance systems and dependence on temporary external assistance. Evidence from Uganda documented established surveillance and rapid-response arrangements alongside persistent facility-level weaknesses, while evidence from the DRC reflected extensive outbreak experience but continuing gaps in healthcare preparedness. Evidence from South Sudan identified substantial infrastructural and humanitarian constraints. However, the uneven geographical and methodological distribution of the evidence precluded direct comparison or ranking of preparedness across the three countries. Sustainable Ebola preparedness requires WASH infrastructure, practical healthcare-worker training, community participation and cross-border coordination to be incorporated into routine health-system planning and financing. Further comparative and country-specific research is required to measure the independent contribution and long-term functionality of WASH interventions.
Downloads
PlumX Statistics
References
Augustin, M. N., & Mbeva, J.-B. K. (2021). Knowledge, attitudes, and behaviors of healthcare professionals at the start of an Ebola virus epidemic. Infectious Diseases Now, 51(1), 50–54. https://doi.org/10.1016/j.medmal.2020.04.010
Baller, A., Padoveze, M. C., Mirindi, P., Hazim, C. E., Lotemo, J., Pfaffmann, J., Ndiaye, A., Carter, S., Chabrat, M.-A. D., Mangala, S., Banzua, B., Umutoni, C., Niang, N. R., Kabego, L., Ouedraogo, A., Houndjo, B., Mwesha, D., Ousman, K. B., Kolwaite, A., . . . Fall, I. S. (2022). Ebola virus disease nosocomial infections in the Democratic Republic of the Congo: A descriptive study of cases during the 2018–2020 outbreak. International Journal of Infectious Diseases, 115, 126–133. https://doi.org/10.1016/j.ijid.2021.11.039
Bepouka, B., & Kafua, M. (2026). Healthcare-associated transmission and early IPC system vulnerabilities during the 2026 Bundibugyo Ebola outbreak in eastern Democratic Republic of the Congo. Antimicrobial Resistance & Infection Control, 15(1), Article 88. https://doi.org/10.1186/s13756-026-01779-8
Biedron, C., Lyman, M., Stuckey, M. J., Homsy, J., Lamorde, M., Luvsansharav, U.-O., Wilson, K., Gomes, D., Omuut, W., Okware, S., Semanda, J. N., Kiggundu, R., Bulwadda, D., Brown, V., Nelson, L. J., Driwale, A., Fagan, R., Park, B. J., & Smith, R. M. (2019). Evaluation of infection prevention and control readiness at frontline health care facilities in high-risk districts bordering Ebola virus disease-affected areas in the Democratic Republic of the Congo—Uganda, 2018. MMWR Morbidity and Mortality Weekly Report, 68(39), 851–854. https://doi.org/10.15585/mmwr.mm6839a4
Bigirimana, T., Gazin, P., Kabamba, K., & Makutu, S. (2020). Prévention et contrôle des infections au cours de la 11e épidémie de la maladie à virus Ebola dans la province de l’Équateur, République démocratique du Congo [Infection prevention and control during the 11th Ebola virus disease outbreak in Equateur Province, Democratic Republic of the Congo]. Bulletin de la Société de Pathologie Exotique, 113(4), 187–189. https://doi.org/10.3166/bspe-2020-0148
Broadhurst, M. J., Ayebare, R. R., Brett-Major, D. M., Carroll, S. M., Lacore, J., Laskey, A. D., Lawler, J. V., Lord, P., Vazquez, R. M., Waitt, P., & Lamorde, M. (2024). Feasibility assessment of a novel isolation care tent in Uganda during the 2022 Sudan ebolavirus outbreak. Health Security, 22(S1), S86–S96. https://doi.org/10.1089/hs.2023.0177
Bwire, G., Sartorius, B., Guerin, P., Tegegne, M. A., Okware, S. I., & Talisuna, A. O. (2023). Sudan Ebola virus outbreak in Uganda, 2022: Lessons learnt and future priorities for sub-Saharan Africa. BMC Medicine, 21, Article 144. https://doi.org/10.1186/s12916-023-02847-1
Checchi, F., Eamer, G., Katshitshi, J., Robles Dios, L., Kai, A., & Warsame, A. (2025). Effect of a safe and dignified burial intervention on Ebola virus transmission in the eastern Democratic Republic of the Congo, 2018–19: A propensity score analysis. The Lancet Global Health. https://doi.org/10.1016/S2214-109X(25)00220-7
Faico-Filho, K. S. (2026). Past lessons for the 2026 Bundibugyo virus outbreak: Filovirus infection prevention in conflict-affected settings. Journal of Hospital Infection, 175, 84–93. https://doi.org/10.1016/j.jhin.2026.06.023
Freeman, A. Y. S., Rumunu, J. P., Modi, Z. A., Guyo, A. G., Achier, A. A. U., Alor, N. A. J., Ochan, T. D. K., Ochan, W. A., Maleghemi, S., Berta, K. K., & Olu, O. O. (2022). Assessment of infection prevention and control readiness for Ebola virus and other disease outbreaks in a humanitarian crisis setting: A cross-sectional study of health facilities in six high-risk states of South Sudan. Pan African Medical Journal, 42(Suppl. 1), Article 10. https://doi.org/10.11604/pamj.supp.2022.42.1.33906
Gulumbe, B. H., Aminu, U., Adesola, R. O., & Lawan, K. A. (2023). Now that the Ebola outbreak in Uganda is over: How do we avert future outbreaks in Africa? Journal of Global Health Science, 5(1), Article e3. https://doi.org/10.35500/jghs.2023.5.e3
Huda, Q., Olu, O., Chungong, S., Yahaya, A. A., Nsenga, N., Conteh, I., et al. (2019). Review of preparedness and readiness for Ebola virus disease in South Sudan by joint monitoring missions: Progress, successes, challenges and the way forward. Weekly Epidemiological Record, 94, xlvi–xlviii. https://iris.who.int/bitstreams/e0e170a2-cd66-4711-8ad9-66e7abcd7e1d/download
Ishida, K., Lozier, M., Medley, A. M., Trinies, V., Hug, C., Ripkey, C., Kesande, M., Tusabe, F., Yapswale, S., Ocitti, F., Isabirye, H., Nanyondo, J., Watsisi, M., Lamorde, M., & Berendes, D. (2024). Changes in access to alcohol-based hand rub and hand hygiene adherence among healthcare workers after a hand-rub production and distribution programme in rural Uganda before and during the COVID-19 pandemic. The American Journal of Tropical Medicine and Hygiene, 111(6), 1343–1352. https://doi.org/10.4269/ajtmh.24-0040
Jombart, T., Zeng, W., Yao, M., Cori, A., Ahuka-Mundeke, S., Samaha, H., Wilkinson, T., Mossoko, M., Lokonga, J.-P., Baabo, D., El Kadiri El Yamini, F., Eozenou, P. H.-V., Yuma, S., & Mobula, L. (2025). Contrasting the impact and cost-effectiveness of successive intervention strategies in response to Ebola in the Democratic Republic of the Congo, 2018–2020. BMJ Global Health, 10(4), Article e015822. https://doi.org/10.1136/bmjgh-2024-015822
Kabego, L., Kourouma, M., Ousman, K., Baller, A., Milambo, J.-P., Kombe, J., Houndjo, B., Boni, F. E., Musafiri, C., Molembo, S., Kalumuna, S., Tshongo, M., Biringiro, J. N., Moke, N., Kumutima, C., Nkita, J., Ngoma, P., Azzouz, C., Okum, R., . . . Fall, I. S. (2023). Impact of multimodal strategies including a pay-for-performance strategy in the improvement of infection prevention and control practices in healthcare facilities during an Ebola virus disease outbreak. BMC Infectious Diseases, 23, Article 12. https://doi.org/10.1186/s12879-022-07956-5
Keita, M., Polonsky, J. A., Ahuka-Mundeke, S., Ilumbulumbu, M. K., Dakissaga, A., Boiro, H., Anoko, J. N., Diassy, L., Ngwama, J. K., Tosalisana, M. K., Nsio, J., Chérif, I. S., Boland, S. T., Delamou, A., Yam, A., Gueye, A. S., Flahault, A., Dagron, S., Keiser, O., & Fall, I. S. (2022). A community-based confinement strategy to reduce the spread of Ebola virus disease: An analysis of the 2018–2020 outbreak in the Democratic Republic of the Congo [Preprint]. Research Square. https://doi.org/10.21203/rs.3.rs-1522670/v1
Lamorde, M., Lozier, M., Kesande, M., Akers, P., Tumuhairwe, O., Watsisi, M., Omuut, W., Person, M., Murphy, J., Quick, R., & Berendes, D. (2020). Access to alcohol-based hand rub is associated with improved hand hygiene in an Ebola-threatened district of western Uganda. Infection Control & Hospital Epidemiology, 41(S1), S457. https://doi.org/10.1017/ice.2020.1130
Ma, Y., Xu, Y., Tan, L., Tian, Y., Ran, J., Zeng, X., Huang, T., & Lu, H. (2026). Imported Ebola as a stress test of hospital resilience in an era of global connectivity. Bioscience Trends, 20(3), 332–339. https://doi.org/10.5582/bst.2026.01141
Mirindi, P., Padoveze, M. C., Mearns, S., Willet, V., Bahatungire, R. R., Katwesigye, E., Mutegeki, M., et al. (2025). WHO infection prevention and control health facility rapid assessment tool for Ebola and Marburg diseases: A validation mixed-method study [Preprint]. medRxiv. https://doi.org/10.1101/2025.08.08.25333289
Muzembo, B. A., Hayashi, C., & Kitahara, K. (2026). Poor preparedness of Congolese healthcare workers for Ebola during the 10th outbreak (2018–2020). Industrial Health, 64(2), 202–210. https://doi.org/10.2486/indhealth.2025-0020
Mwamba, D. K., Akilimali, P. Z., Manianga, C., Kapanga, S., Ngombe, N. K., Shonganye, J., Angendu, K. B., Moullec, G., & Zarowsky, C. (2026). Current experiences and practices of surveilling and managing Ebola virus disease outbreaks in the Democratic Republic of Congo by involving the community in a “One Health” approach. Pandemics, 1(1), Article 3. https://doi.org/10.3390/pandemics1010003
Mwamba, D. K., Angendu, K. B., Diouf, W., Mikobi, M.-C., Leonard, O., Kalala, D., Ntumba, N., Kakule, D., Kayembe, D. K., Sana, E., Kabasele, B., Katya, J., Montoyo, A., Serra, B., Bulambo, H., Otshudiema, J., Kapanga, S., Balayulu, O., Muya, J., . . . Akilimali, P. Z. (2025). Seven strategies implemented in response to the 16th Ebola virus disease outbreak in the Democratic Republic of Congo: Lessons learned over a three-month period. Viruses, 18(1), Article 28. https://doi.org/10.3390/v18010028
Nabatanzi, S., Muruta, A. N., Makumbi, I., Ario, A. R., Kwesiga, B., Kagirita, A. A., Bulage, L., Lubwama, B., Gonahasa, D. N., Mbaka, P., Komakech, I., Nabatanzi, M., Ocom, F., Homsy, J., Brown, V., Ojwang, J., Matseketse, D., Ameda, I. M., Kyazze, S., . . . Nanyunja, M. (2024). Review of Uganda’s high-risk Ebola preparedness activities enabling prompt control of an imported Ebola virus outbreak from the Democratic Republic of the Congo, June 2019. Journal of Interventional Epidemiology and Public Health, 7(2), Article 7. https://doi.org/10.37432/jieph.2024.7.2.114
Nabwami, P. E., Nyaberi, J. M., Monyangi, N. N., Nantima, N., Kayiwa, J., & Mokaya, A. G. (2025). Preparedness of healthcare workers for the Ebola outbreak in Mubende and Kassanda districts, Uganda. Journal of Public Health in Africa, 16(4), Article 1347. https://doi.org/10.4102/jphia.v16i4.1347
Nanyondo, S. J., Nakato, S., Franklin, J., Kwiringira, A., Malikisi, M., Kesande, M., Wailagala, A., Suubi, R., Byonanebye, D. M., Katwesigye, E., Katongole, P., Kasule, J., Bayo, L. B., Kasendwa, M., Musisi, D., Hunter, J., Oakley, L. P., Dennison, C., Ndegwa, L., . . . Lamorde, M. (2025). Implementation of an infection prevention and control response strategy to combat the Sudan virus disease outbreak in an urban setting, the Kampala metropolitan area, Uganda, 2022. BMC Infectious Diseases, 25, Article 317. https://doi.org/10.1186/s12879-025-10720-0
Nicastri, E., Kobinger, G., Vairo, F., Montaldo, C., Mboera, L. E. G., Ansunama, R., Zumla, A., & Ippolito, G. (2019). Ebola virus disease: Epidemiology, clinical features, management, and prevention. Infectious Disease Clinics of North America, 33(4), 953–976. https://doi.org/10.1016/j.idc.2019.08.005
Ninsiima, M., Migisha, R., Ndyabakira, A., Katana, E., Aanyu, D., Kabami, Z., Zalwango, J. F., Naiga, H. N., Kiggundu, T., Agaba, B., Zavuga, R., Kizito, S. N., Zalwango, M. G., King, P., Wanyana, M. W., Simbwa, B. N., Kawungezi, P. C., Akunzirwe, R., Nakamya, P., . . . Ario, A. R. (2025). Strategies utilized during Sudan virus disease outbreak response in Kampala City, Uganda, 2022–2023. Journal of Epidemiology and Global Health, 15, Article 92. https://doi.org/10.1007/s44197-025-00438-2
Okamoto, F., Nishikawa, Y., Twalla, Y., Nakayama, T., & Masai, S. (2022). Ebola outbreak in Uganda: Patient values and preferences. The Lancet, 400(10367), 1925–1926. https://doi.org/10.1016/S0140-6736(22)02321-2
Okoror, L., Kapona, O., Kasonde, M., Tobin, A. E., Asogun, D., & Otukoya, A. M. (2020). Ebola virus disease preparedness in countries bordering the Democratic Republic of Congo: Lessons from the West African outbreak. African Journal of Infectious Diseases, 14(2), 36–41. https://doi.org/10.21010/ajid.v14i2.6
Olu, O. O., Lako, R., Wamala, J. F., Ramadan, P. O., Ryan, C., Udenweze, I., Berta, K., Guyo, A. G., Sokemawu, A., Tukuru, M., Gray, H. J., & Chimbaru, A. (2020). What did we learn from preparing for cross-border transmission of Ebola virus disease into a complex humanitarian setting—The Republic of South Sudan? Infectious Diseases of Poverty, 9, Article 40. https://doi.org/10.1186/s40249-020-00657-8
Olum, R., Ahaisibwe, B., Atuhairwe, I., Balizzakiwa, T., Kizito, P. M., Apiyo, M., Kalanzi, J., Nabawanuka, A., Bahatungire, R., & Kerry, V. (2024). Readiness to manage Ebola virus disease among emergency healthcare workers in Uganda: A nationwide multicenter survey. BMC Health Services Research, 24, Article 1271. https://doi.org/10.1186/s12913-024-11741-2
Oposhia, J., Kungu, J. M., Nantamu, P. D., Namayanja, J., Okuyo, C. A. B., Mulowoza, M., Kabali, K., Edson, K., & Olupot-Olupot, P. (2026). Ebola virus disease preparedness in subnational health systems: A readiness assessment of Jinja District, Uganda. Journal of Tropical Medicine, 2026, Article 5519966. https://doi.org/10.1155/jotm/5519966
Ousman, K., Kabego, L., Talisuna, A., Diaz, J., Mbuyi, J., Houndjo, B., Ngandu, J.-P., Omba, G., Aruna, A., Mossoko, M., Djingarey, M. H., Balde, T., Abok, P., Diallo, B., Dovlo, D., Yao, M., Fortin, A., Formenty, P., & Fall, I. S. (2019). The impact of infection prevention and control bundle implementation on IPC compliance during the Ebola virus outbreak in Mbandaka, Democratic Republic of the Congo: A before-and-after design. BMJ Open, 9(9), Article e029717. https://doi.org/10.1136/bmjopen-2019-029717
Ousman, K., Thumath, M., McKay, G., Niyukuri, D., Ombeni, D. M., Kabego, L., Banzua, B., Mangala, S., Tatabod, R., Mirindi, P. N., Talisuna, A., Houndjo, B., Kieta, M., & Baller, A. (2022). Evaluation of a health systems strengthening intervention to improve Ebola virus disease infection prevention and control in the Democratic Republic of the Congo. Journal of Global Health Reports, 6, Article e2022037. https://doi.org/10.29392/001c.36558
Peters, M. D. J., Godfrey, C., McInerney, P., Munn, Z., Tricco, A. C., & Khalil, H. (2020). Scoping reviews. In E. Aromataris and Z. Munn (Eds.), JBI manual for evidence synthesis. JBI. https://doi.org/10.46658/JBIMES-20-12
Rethlefsen, M. L., Farrell, A. M., Osterhaus Trzasko, L. C., & Brigham, T. J. (2022). Librarian co-authors correlated with higher quality reported search strategies in general internal medicine systematic reviews. Journal of Clinical Epidemiology, 145, 1–9. https://doi.org/10.1016/j.jclinepi.2022.01.025
Rethlefsen, M. L., Kirtley, S., Waffenschmidt, S., Ayala, A. P., Moher, D., Page, M. J., Koffel, J. B., Blunt, H., Brigham, T., Chang, S., Clark, J., Conway, A., Couban, R., de Kock, S., Farrah, K., Fehrmann, P., Foster, M., Fowler, S. A., Glanville, J., . . . Young, S. (2021). PRISMA-S: An extension to the PRISMA statement for reporting literature searches in systematic reviews. Systematic Reviews, 10, Article 39. https://doi.org/10.1186/s13643-020-01542-z
Sikakulya, F. K., Mulisya, O., Munyambalu, D. K., & Bunduki, G. K. (2020). Ebola in eastern Democratic Republic of the Congo: One Health approach to infectious disease control. One Health, 9, Article 100117. https://doi.org/10.1016/j.onehlt.2019.100117
Tricco, A. C., Lillie, E., Zarin, W., O’Brien, K. K., Colquhoun, H., Levac, D., Moher, D., Peters, M. D. J., Horsley, T., Weeks, L., Hempel, S., Akl, E. A., Chang, C., McGowan, J., Stewart, L., Hartling, L., Aldcroft, A., Wilson, M. G., Garritty, C., . . . Straus, S. E. (2018). PRISMA extension for scoping reviews (PRISMA-ScR): Checklist and explanation. Annals of Internal Medicine, 169(7), 467–473. https://doi.org/10.7326/M18-0850
World Health Organization. (2019a). A package for monitoring operational indicators of the Ebola virus disease response in the Democratic Republic of the Congo. https://iris.who.int/items/7be65a23-3cdc-4dcd-8cca-537d53941e68
World Health Organization. (2019b). Operational readiness and preparedness for Ebola virus disease in countries neighbouring the Democratic Republic of the Congo. https://iris.who.int/items/f1143321-21dc-42c8-b1b1-a2474c3b4b2b
World Health Organization. (2019c). Review of preparedness and readiness for Ebola virus disease in South Sudan by joint monitoring missions: Progress, successes, challenges and the way forward. https://iris.who.int/items/4a1b6f7e-5a39-40a0-91bb-21e7ea19053c
World Health Organization. (2023). Infection prevention and control guideline for Ebola and Marburg disease, August 2023. https://iris.who.int/handle/10665/372261
World Health Organization. (2024). Ebola and Marburg disease outbreaks: Infection prevention and control research priorities in health care settings. https://iris.who.int/items/8c201433-55b2-4d7a-be10-612fabfe76d9
World Health Organization. (2025, April 24). Ebola disease. https://www.who.int/news-room/fact-sheets/detail/ebola-disease
World Health Organization. (2026). Infection prevention and control guideline for Ebola and Marburg diseases. https://iris.who.int/handle/10665/385879
Yang, J., Claude, K. M., Kimani, E., & Hawkes, M. T. (2026). Limited durability of improvements in infection prevention and control practices following reactive interventions leaves healthcare facilities vulnerable to Ebola virus transmission. Clinical Infectious Diseases, 83(1), e130–e132. https://doi.org/10.1093/cid/ciag192
Zeng, W., Samaha, H., Yao, M., Ahuka-Mundeke, S., Wilkinson, T., Jombart, T., Baabo, D., Lokonga, J.-P., Yuma, S., & Mobula-Shufelt, L. (2023). The cost of public health interventions to respond to the 10th Ebola outbreak in the Democratic Republic of the Congo. BMJ Global Health, 8(10), Article e012660. https://doi.org/10.1136/bmjgh-2023-012660
Copyright (c) 2026 James Arike Charles Duku

This work is licensed under a Creative Commons Attribution 4.0 International License.


