APPLICATION OF RESPONSE SURFACE METHODOLOGY IN MEDIUM COMPONENTS OPTIMIZATION TO ENHANCE SERRATIOPEPTIDASE PRODUCTION BY STREPTOMYCES HYDROGENANSMGS13

Abstract

For enhanced production of Serratiopeptidase by an actinomycetestrain, Streoptomyces hydrogenans MGS13, optimization of fermentationmedium was initially carried out by conventional method of ‗one-factor-at-atime‘.Later it was optimized by applying response surface methodology.Interactions were studied with four variables viz. levels of dextrose, soyabean meal and inoculum & pH using Central Composite Design. This modelwas validated by conducting the experiments under the optimized conditions,which resulted in the improved Serratiopeptidase production of 254.56IU/mL (Predicted response 278.087 IU/mL), thus proving the validity of themodel. Streptomyces hydrogenans MGS13 strain isolated from mangrovesoil sediment was taken up for this study. This study demonstrates the abilityof the strain, Streptomyces hydrogenans MGS13 for the Serratiopeptidaseproduction and the application of response surface methodology withimproved Serratiopeptidase production. The statistical experimental design issimple and less time consuming & is adequate to economize thefermentation. This is the first report on the application of response surfacemethodology for Serratiopeptidase production by an actinomycete isolate.