SMOOTH TEMPERATURE DECREASING FOR NITROGEN REMOVAL IN COLD (9-15° C) ANAMMOX BIOFILM REACTOR TESTS

Abstract

For N-rich wastewater treatment the anaerobic ammonium oxidation (anammox) and nitritation-anammox (deammonification) processes are widely used. In a deammonification moving bed biofilm reactor (MBBR) a maximum total nitrogen removal rate (TNRR) of 1.5 g N m-2d-1(0.6 kg N m-3d-1) was achieved. During biofilm cultivation, temperature was gradually lowered by 0.5° C per week, and a similar TNRR was sustained at 15° C. qPCR analysis showed an increase in Candidatus Brocadia quantities from 5×103 to 1×107 anammox gene copies g-1 TSS despite temperature lowered to 15° C. Fluctuations in TNRR were rather related to changes in influent NH4+ concentration. To study the short-term effect of temperature on the TNRR, a series of batch-scale experiments were performed which showed sufficient TNRRs even at 9-15° C (4.3-5.4 mg N L-1 h-1, respectively) with anammox temperature constants ranging 1.3-1.6. After biomass was adapted to 15° C, the decrease in TNRR in batch tests at 9° C was lower (15-20%) than for biomass adapted to 17-18° C. Our experiments show that a biofilm of a deammonification reactor adapted to 15° C successfully tolerates shortterm cold shocks down to 9° C retaining a high TNRR.