GENETIC DIVERSITY OF FABA BEAN (VICIA FABA L.) POPULATIONS ESTIMATED BY ISOZYMIC AND MOLECULAR MARKERS: RELATIONSHIP BETWEEN THE TWO METHODS

  • Ali Ouji Regional Research Development Office of Agriculture in Semi Arid North West of Kef, Tunisia Field Crop’s Laboratory, National Institute for Agricultural Research of Tunisia, Ariana, Tunisia
  • Safia El-Bok Laboratory of Biodiversity, Climate Change and Biotechnology, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis, Tunisia.
  • Mustapha Rouaissi Biotechnology and Physiology Laboratory, National Institute for Agricultural Research of Tunisia, Ariana, Tunisia
  • Naeem H. Syed Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD25DA, UK
  • Maria Jose Suso Instituto de Agricultura Sostenible, Apartado, Cordoba, España
  • Andrew J. Flavell Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD25DA,
  • Mohamed El Gazzah Laboratory of Biodiversity, Climate Change and Biotechnology, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis, Tunisia
  • Mongi Ben Younes Regional Research Development Office of Agriculture in Semi Arid North West of Kef, Tunisia
  • Mohamed Kharrat Field Crop’s Laboratory, National Institute for Agricultural Research of Tunisia, Ariana, Tunisia

Abstract

In our previous studies, the genetic diversity among nine Tunisian faba bean (Vicia faba) populations was analysed using isozymes and sequence-specific amplification polymorphism (SSAP) markers. The objectives of this study were to compare the application and utility of isozymes and sequence-specific amplification polymorphism (SSAP) techniques for analysis of genetic diversity among nine Tunisian faba bean (Vicia faba) populations. A high genetic diversity within populations was detected by both isozymes (SOD, 6-PGD, ME, EST, SKDH, FDH and GDH) and (SSAP) markers (PDR1, Tps19 and Tvf4). For all populations, the genetic diversity revealed by SSAP was more pronounced than that detected with isozyme, based on polymorphic profiles. The analyses of correspondance between the tow methodes based in Mantel test revealed a low correlation ( r=0.177). The low correspondance indicated the absence of correlation and therfore the complimentarity

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Published
2015-11-28
How to Cite
Ouji, A., El-Bok, S., Rouaissi, M., Syed, N. H., Suso, M. J., Flavell, A. J., El Gazzah, M., Younes, M. B., & Kharrat, M. (2015). GENETIC DIVERSITY OF FABA BEAN (VICIA FABA L.) POPULATIONS ESTIMATED BY ISOZYMIC AND MOLECULAR MARKERS: RELATIONSHIP BETWEEN THE TWO METHODS. European Scientific Journal, ESJ, 11(33). Retrieved from https://eujournal.org/index.php/esj/article/view/6662